Jean-François Lesgards

 Doctor in Chemistry-Biochemistry

Nutrition – Environment – Health – Oxidative Stress/Inflammation 

                                                                      

EDUCATION AND DIPLOMAS

• • 2000 : PhD (Chemistry-Biochemistry) – University of Aix-Marseille-  Title : “Contribution to the determination of antioxidant status in humans. Chemical and biochemical aspects.“ Laboratory: Mediterranean Institute of Nutrition. 

• • 1996 : DEA in organic chemistry , option : biochemistry – University of Aix-Marseille. 

PROFESSIONAL REFERENCES -25 YEARS OF EXPERIENCE

 Chef Science Officier at Protelicious company [2016/Today]

• • • Independant Scientific advisor and Research and development [2011/2022]

    • Co-head R and for Evergreen Land company (natural insect repellents, health). 

    • Other contracts in health, nutrition and agri-food. 

    • Resarch associate in Laboratoire Sondes Moléculaires en Biologie et Stress Oxydant (SMBSO), Institut de Chimie Radicalaire (ICR), UMR CNRS 7273, Aix Marseille University : Cardiovascular and cancer.

    • Collaboration with Health and Environnement Association, France (ASEF) 

INRA, LABORATORY OF HUAN NUTRITION UMR 1019, Clermont Ferrand-Theix. [2010/2011]

• • • Research ingenior
    • Study of the effects of different protein fractions of milk on urinary metabolomic in obese subjects

IMR, FRE3083 CNRS and Biosciences ISM2, Paul Cézanne University, Marseille. [2009/2010]

• • • Research ingenior
    • Study of the effect of carbonyl stress in diabetes. Enzymatic inhibition, insulin secretion and glucose metabolism

DIABETE AND OBESITY CENTER – Division of Molecular Cardiology, University of Louisville, Kentucky, USA (Dr Aruni Bhatnagar).[2008/2009]

• • • Post doctoral position and co-head of Analytical Chemistry department
    • Study of the detoxification pathways of acrolein and its toxicity in heart diseases. Development of analytical methods for the measurement of 3-hydroxypropylmercapturicacid (HPMA) in urine (GC- MS), glutathione-aldehydes adducts in hearts tissues (LC, ESI-MS) in Glutathione Transferase (P) knockout mice

HEART INSTITUTE OF MONTREAL, Qc, Canada. Laboratory of Intermediary Metabolism (Dr Christine des Rosiers). [2005/2008]

• • • Post doctoral position
    • 1/ Study of 4-hydroxynonenal (4-HNE) adducts on blood and tissue proteins in cardiology
    • 2/ Development of a method for the determination of O-linked N- acetyl-glucosamine (hexosamines pathway) on proteins in heart homogenates from rats perfused ex-vivo

SYNCROSOME COMPANY, Marseille (Contract Research Organization, in vivo pharmacology). Experimentation, research and Gdevelopment. Areas: cardiology, gastro-enterology, chemiotherapy [2002/2004]

• • Studies as Independent worker in collaboration with companies (Syncrosome and Yelen, Marseille) and the Regional Chamber of Agriculture. Dietary antioxidants (polyphenols, vitamins), valorisation of Mediterranean diet, antioxidant activity of drugs [2000/2002]

EUROPEAN CENTER OF RESEARCH AND ANALYSIS (CEDRA), Dijon. Contribution to the development of a biological test for monitoring the global effectiveness of antioxidant systems (KRL test) [1997/2000]

 

REFERENCES

• • (1) Lesgards JF, Cerdan D, Perronne C, Sabatier JM, Azalbert X, Rodgers EA, McCullough PA. Toxicity of SARS-CoV-2 Spike Protein with Respiratory Infection and Produced from COVID-19 mRNA and Adenoviral DNA Vaccines. Archives of Microbiology and Immunology. 7 (2023): 121-138. 

  • (2) Lesgards JF. Benefits of Whey Proteins on Type 2 Diabetes Mellitus Parameters and Prevention of Cardiovascular Diseases. Nutrients 15(5):1294 (2023) 

  • (3) Vidal N, Lesgards JF. WO2019193561 (A2) – INSECT-REPELLENT COMPOSITION COMPRISING ONE OR MORE INSECT-REPELLENT FATTY ACIDS WITH FROM 9 TO 21 CARBON ATOMS (2019) 

  • (4) Vidal N, Lesgards JF. TR201901909 (T4) – AQUEOUS FORMULATION COMPRISING A LIPOPHILIC COMPOSITION COMPRISING CELLULOSE AND CLAY (2019) 

  • (5) Lesgards JF, Baldovini N, Vidal N, Pietri S. Anticancer activities of essential oils constituents and synergy with conventional therapies: a review. Phytother Res. (10):1423-46 (2014). 

  • (6) Lesgards JF, Gauthier C, Iovanna J, Vidal N, Dolla A, Stocker P. Effect of reactive oxygen and carbonyl species on crucial cellular antioxidant enzymes. Chem Biol Interact. 190, 28-34 (2011). 

  • (7) Des Rosiers C, Lesgards JF, Tadif JC. Patent No US 2010/0330683 A1. Method for quantifying oxidative stress caused by different pathways. Dec 30 (2010). 

  • (8) Conklin DJ, Barski OA, Lesgards JF, Juvan P, Rezen T, Rozman D, Prough RA, Vladykovskaya E, Liu S, Srivastava S, Bhatnagar A. Acrolein consumption induces systemic dyslipidemia and lipoprotein modification. Toxicol Appl Pharmacol., 243, 1-12 (2010). 

  • (9) Conklin DJ, Haberzettl P, Lesgards JF, Prough RA, Srivastava S, Bhatnagar A. Increased Sensitivity of Glutathione S-transferase P-null Mice to Cyclophosphamide-induced Urinary Bladder Toxicity. J Pharmacol Exp Ther., 331, 456-69 (2009). 

  • (10) Jean-François Lesgards, Isabelle Robillard Frayne, Blandine Comte and Christine Des Rosiers Differential distribution of 4-hydroxynonenal adducts to sulfur and nitrogen residues in blood proteins as revealed using Raney nickel and gas chromatography-mass spectrometry. Free Radical Biology and Medicine., 47, 1375-85 (2009). 

  • (11) Djeridane A, Yousfi M, Nadjemi B,Vidal N, , Lesgards JF, Stocker P. Screening of some Algerian medicinal plants for the phenolic compounds and their antioxidant activity. European Food Research and Technology, 224, 6, 801-809 (2007). 

  • (12) Lesgards JF, Lehucher-Michel MP, Vidal N, Prost M, Stocker P. Assessment of antioxidative activity of water-soluble and lipo-soluble vitamins in human whole blood. Comparative analysis between a biological test and chemical methods. International Journal of Vitamin and Nutrition Research, 75, 11-18 (2005). 

  • (13) Stocker P, Lesgards JF, Vidal N, Challier F, Prost M. ESR study of a biological assay on whole blood: antioxidant efficiency of various vitamins. Biochim. Biophys. Acta., 1621, 1-8 (2003). 

  • (14) Lesgards JF, Durand P Lassarre M, Lanteaume A, Lesgards G, Durand P, Prost M, Lehucher-Michel MP. Assessment of lifestyle effects on the overall antioxidant capacity of healthy subjects. Environ Health Perspect., 110, 479-86 (2002). 

  • (15) Lesgards JF, Prost M, Lehucher-Michel MP. Oxidative stress and its implications. Myalgies International, 2, 14-19 (2002). Review in French. 

  • (16) Lehucher-Michel MP, Lesgards JF, Delubac O, Stocker P, Durand P, Prost M. Oxidative stress and human disease. Current knowledge and perspectives for prevention. Presse Med., 30, 1076-81 (2001). Review in French. 

  • (17) Stocker P, Lesgards JF, Lehucher-Michel MP, Raffi J, Prost M, Lesgards G. Hemolysis test: approach of the radical mechanisms. Characterization of the antioxydant capacity of vitamins. Ann. Fals. Exp. Chim., 949, 471-483 (2000). Review in French.